|
KMID : 0043320090320111515
|
|
Archives of Pharmacal Research
2009 Volume.32 No. 11 p.1515 ~ p.1523
|
|
|
Sanjoinine A Isolated from Semen Zizyphi Spinosi Protects Against Kainic Acid-Induced Convulsions
|
|
Oh Ki-Wan
Yoon Sung-Ryul
Jo Young-Jun
Yang Shulong
Kim Yun-Bae
Nam Sang-Yoon
Kim Hyoung-Chun
Hong Jin-Tae
|
|
|
Abstract
|
|
|
|
These experiments were performed to know whether sanjoinine A, a component of the alkaloid fraction of Semen Zizyphi Spinosi, acts as an anti-convulsive agent in the kainic acid (KA)-induced experimental convulsion model and whether these effects are mediated by decreased intracellular calcium. Oral administration of sanjoinine A (4 and 8 mg/kg) increased the survival rate and latency of convulsion onset, and decreased the seizure scores and the weight loss induced by intraperitoneal (i.p.) injection of KA (50 mg/kg) in mice. In addition, sanjoinine A protected against neuronal damage and apoptosis in the hippocampus after KA administration, as analyzed by using immunohistochemistry and TUNEL assay. Sanjoinine A also significantly blocked seizure-form electroencephalogram alterations induced by KA. Moreover, in cultured rat neuronal cells, sanjoinine A inhibited KA-induced cell death, as measured by propidium iodide detection. Sanjoinine A also increased intracellular chloride and inhibited the elevation of intracellular calcium induced by KA. Sanjoinine A, therefore protects against KAinduced convulsions by increasing intracellular chloride and reducing intracellular calcium levels.
|
|
|
KEYWORD
|
|
|
Sanjoinine A, Kainic acid, Convulsion, Cell death, Electroencephalogram, Calcium level
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|